19RW
(University of New England)
Collection:
A small sample of leaf material (~0.02g) was collected by Mr Russell Wait from plants cultivated in a private garden (Riddells Creek, VIC). Specimens were immediately placed in glass vials containing a small volume of MeOH and shipped to the lab where MeOH was added to make up to ~ 1 mL. Samples were left in the solvent overnight to ~24 h. Samples were diluted with further MeOH and/or filtered through cotton wool, as necessary. Specimens were then injected into the GC-MS for characterization, or frozen until characterization.
GC–MS:
Agilent GC System 7890A; inert MSD with triple-axis detector 7975C; Agilent HP-5MS column (30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min). To limit the impact of solvent-related peaks, peaks prior to a retention time of 3.5 min were not considered in analyses.
Analysis:
Spectra were matched to the NIST11 library and a small private Eremophila compound library maintained at the University of New England. The top 10 peaks were selected, and arithmetic (retention) indices (AI) calculated and compared to the NIST Webbook (http://webbook.nist.gov/chemistry/). An annotation was rejected if the AI (+/-2%) did not match published values for nonpolar column data. If the quality match of the spectra to the NIST11 or private library was under 80% the annotation was rejected. Where no published AI was available, a compound was accepted if the quality of the spectra match was 90% or over.
19RW:365
AI 1926
5%
5%
8%
10%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:322
AI 1175
38%
4%
3%
15%
4%
3%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:375
AI 1660
3%
4%
4%
11%
7%
4%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:376
AI 934
3%
9%
13%
4%
3%
5%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:347
AI 934
8%
9%
2%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:310
AI 1425
11%
3%
16%
2%
4%
3%
3%
3%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:318
AI 974
5%
6%
22%
7%
3%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:330
AI 1294
3%
2%
5%
4%
18%
8%
6%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:308
AI 1315
4%
8%
9%
5%
2%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:314
3%
2%
2%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:364
AI 960
14%
14%
4%
3%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:321
14%
2%
3%
3%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:333
AI 949
10%
5%
4%
4%
13%
5%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:348
AI 907
6%
19%
2%
7%
3%
2%
7%
29%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:317
AI 949
5%
2%
7%
3%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:319
AI 1090
7%
14%
2%
5%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:326
9%
5%
4%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:352
AI 934
14%
5%
2%
4%
3%
2%
4%
41%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:371
AI 1519
2%
4%
3%
6%
29%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:377
AI 934
10%
4%
7%
6%
6%
12%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:334
9%
8%
4%
3%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:331
AI 1488
10%
6%
2%
24%
5%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:336
AI 1925
6%
10%
6%
4%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:357
AI 906
7%
7%
30%
5%
3%
2%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:345
AI 934
33%
9%
2%
7%
2%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:361
AI 906
8%
2%
43%
3%
3%
2%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:312
4%
3%
2%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:368
4%
3%
4%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:358
AI 906
5%
34%
2%
5%
3%
5%
2%
8%
3%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:374
AI 1958
4%
2%
3%
5%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:309
4%
5%
23%
9%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:313
AI 1030
3%
3%
6%
6%
4%
17%
4%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:315
AI 934
5%
5%
5%
18%
6%
4%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:316
AI 907
5%
19%
2%
4%
4%
3%
3%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:320
AI 934
2%
4%
8%
31%
7%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:323
3%
4%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:324
AI 934
7%
4%
4%
3%
3%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:350
AI 934
8%
2%
1%
2%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:325
AI 934
30%
3%
10%
4%
4%
4%
3%
3%
4%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:329
13%
4%
3%
2%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:327
AI 906
4%
3%
50%
5%
2%
2%
2%
6%
3%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:328
AI 934
4%
4%
4%
3%
2%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:332
AI 907
3%
45%
2%
2%
3%
4%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:366
13%
49%
3%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:311
AI 934
4%
22%
4%
3%
7%
3%
4%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:337
AI 934
7%
8%
3%
4%
9%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:343
AI 907
2%
16%
3%
3%
11%
5%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:359
AI 1925
10%
3%
25%
7%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:373
AI 1925
6%
4%
22%
4%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:335
AI 949
8%
3%
3%
3%
3%
14%
3%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:338
AI 978
2%
9%
4%
44%
5%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:340
AI 991
4%
1%
7%
7%
58%
6%
4%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:341
AI 934
17%
4%
14%
5%
2%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:342
AI 934
9%
5%
2%
22%
5%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:344
AI 934
2%
2%
1%
9%
1%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:346
AI 1926
5%
16%
6%
2%
4%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:349
AI 906
3%
33%
3%
3%
12%
3%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:351
AI 978
3%
3%
6%
4%
3%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:353
AI 1315
4%
6%
7%
3%
6%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:354
AI 949
8%
10%
3%
2%
6%
3%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:355
5%
3%
2%
3%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:356
AI 934
6%
5%
12%
23%
5%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:362
AI 934
34%
6%
6%
5%
4%
8%
3%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:363
AI 1006
1%
9%
13%
4%
3%
10%
2%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:367
AI 1424
20%
3%
29%
1%
19%
2%
2%
2%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:381
8%
6%
3%
4%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:369
AI 1926
5%
25%
7%
3%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:370
AI 906
2%
14%
8%
4%
32%
3%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:372
AI 934
10%
5%
9%
2%
33%
3%
9%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:380
AI 934
3%
3%
4%
7%
6%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
19RW:382
AI 974
3%
5%
6%
3%
5%
10%
3%
12%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
