18E
(University of New England)
Collection:
A small sample of leaf material (~0.02g) was collected from plants cultivated at the Australian Arid Lands Botanic Garden (Port Augusta, SA) by staff at the garden. Specimens were immediately placed in glass vials containing a small volume of MeOH and shipped to the lab where MeOH was added to make up to ~ 1 mL. Samples were left in the solvent overnight to ~24 h. Samples were diluted with further MeOH and/or filtered through cotton wool, as necessary. Specimens were then injected into the GC-MS for characterization, or frozen until characterization.
GC–MS:
Agilent GC System 7890A; inert MSD with triple-axis detector 7975C; Agilent HP-5MS column (30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min). To limit the impact of solvent-related peaks, peaks prior to a retention time of 3.5 min were not considered in analyses.
Analysis:
Spectra were matched to the NIST11 library and a small private Eremophila compound library maintained at the University of New England. The top 10 peaks were selected, and arithmetic (retention) indices (AI) calculated and compared to the NIST Webbook (http://webbook.nist.gov/chemistry/). An annotation was rejected if the AI (+/-2%) did not match published values for nonpolar column data. If the quality match of the spectra to the NIST11 or private library was under 80% the annotation was rejected. Where no published AI was available, a compound was accepted if the quality of the spectra match was 90% or over.
18E:148
17%
4%
5%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:140
AI 935
32%
23%
11%
11%
15%
1%
1%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:142
AI 907
5%
17%
5%
5%
18%
10%
9%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:143
AI 935
6%
22%
4%
36%
1%
2%
5%
6%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:144
AI 1372
4%
2%
17%
5%
4%
29%
5%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:145
AI 950
3%
7%
3%
54%
6%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:146
2%
3%
2%
7%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:147
AI 978
7%
6%
6%
3%
11%
4%
20%
6%
3%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:149
AI 935
4%
2%
3%
21%
35%
1%
3%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:150
AI 935
2%
20%
1%
44%
3%
2%
2%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:151
AI 1030
2%
58%
2%
16%
4%
2%
4%
2%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:153
AI 907
2%
4%
10%
4%
17%
2%
6%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:155
AI 907
3%
2%
9%
2%
3%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:156
AI 935
53%
5%
3%
2%
13%
2%
2%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:157
AI 1529
4%
27%
2%
29%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:161
AI 1503
9%
5%
7%
4%
32%
4%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:162
AI 1529
3%
24%
3%
23%
10%
2%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:163
AI 935
17%
2%
2%
10%
3%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:165
AI 1176
46%
3%
3%
2%
10%
1%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:166
AI 935
9%
6%
2%
3%
1%
8%
7%
47%
8%
1%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:167
AI 935
29%
5%
5%
8%
5%
3%
12%
5%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:168
AI 935
11%
6%
6%
5%
3%
4%
25%
4%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:169
AI 935
8%
1%
2%
4%
3%
24%
2%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:170
AI 935
25%
8%
4%
5%
7%
5%
20%
4%
4%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:171
AI 907
4%
17%
5%
6%
3%
8%
5%
26%
5%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:174
AI 935
1%
1%
3%
13%
5%
59%
12%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:175
AI 935
19%
2%
9%
3%
2%
9%
3%
12%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:178
AI 1487
4%
2%
2%
8%
2%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:182
AI 1100
2%
2%
7%
4%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:183
AI 1503
19%
2%
4%
4%
11%
7%
20%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:184
AI 935
8%
10%
13%
8%
3%
3%
12%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:185
AI 935
6%
5%
22%
4%
2%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:186
AI 907
12%
3%
49%
4%
3%
3%
2%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:187
AI 935
2%
3%
2%
3%
2%
10%
5%
54%
3%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:188
AI 950
8%
5%
8%
4%
28%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:189
AI 928
6%
16%
13%
1%
1%
18%
39%
1%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:190
AI 935
2%
40%
7%
12%
4%
2%
2%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:191
1%
1%
4%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:192
4%
8%
4%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:195
AI 979
1%
3%
1%
18%
1%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:197
AI 935
11%
20%
3%
5%
3%
2%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:198
AI 948
4%
3%
8%
7%
4%
46%
9%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:199
AI 935
19%
6%
5%
5%
2%
3%
5%
11%
14%
13%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:201
AI 935
21%
2%
11%
1%
12%
7%
1%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:202
AI 935
3%
3%
4%
4%
6%
47%
12%
3%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:203
AI 979
5%
6%
5%
23%
20%
6%
3%
12%
2%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:204
77%
1%
4%
8%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:206
AI 1426
2%
7%
3%
2%
23%
2%
4%
4%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.
18E:207
AI 935
2%
5%
2%
9%
28%
3%
5%
GC: HP-5MS; 30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min.