EremoChem Projects

Collection:
A small sample of leaf material (~0.02g) was collected from plants cultivated at a private Eremophila garden in Inverell (NSW).  Plant identification was confirmed by the garden's horticulturist.  Specimens were immediately placed in glass vials containing ~ 1 mL MeOH.  After overnight extraction, batches were injected into the GC-MS for characterization, or frozen until characterization.  Samples were filtered and/or diluted with further MeOH as necessary.

GC–MS:
Agilent GC System 7890A; inert MSD with triple-axis detector 7975C; Agilent HP-5MS column (30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min). To limit the impact of solvent-related peaks, peaks prior to a retention time of 3.5 min were not considered in analyses.

Analysis:
Spectra were matched to the NIST11 library and a small private Eremophila compound library maintained at the University of New England. The top 10 peaks were selected, and arithmetic (retention) indices (AI) calculated and compared to the NIST Webbook (http://webbook.nist.gov/chemistry/).  An annotation was rejected if the AI (+/-2%) did not match published values for nonpolar column data.  If the quality match of the spectra to the NIST11 or private library was under 80% the annotation was rejected. Where no published AI was available, a compound was accepted if the quality of the spectra match was 90% or over. 

Collection:
A small sample of leaf material (~0.02g) was collected from plants cultivated at the Australian Arid Lands Botanic Garden (Port Augusta, SA) by staff at the garden.  Specimens were immediately placed in glass vials containing a small volume of MeOH and shipped to the lab where MeOH was added to make up to ~ 1 mL.  After overnight extraction, batches were injected into the GC-MS for characterization, or frozen until characterization.  Samples were filtered and/or diluted with further MeOH as necessary.

GC–MS:
Agilent GC System 7890A; inert MSD with triple-axis detector 7975C; Agilent HP-5MS column (30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min). To limit the impact of solvent-related peaks, peaks prior to a retention time of 3.5 min were not considered in analyses.

Analysis:
Spectra were matched to the NIST11 library and a small private Eremophila compound library maintained at the University of New England. The top 10 peaks were selected, and arithmetic (retention) indices (AI) calculated and compared to the NIST Webbook (http://webbook.nist.gov/chemistry/).  An annotation was rejected if the AI (+/-2%) did not match published values for nonpolar column data.  If the quality match of the spectra to the NIST11 or private library was under 80% the annotation was rejected. Where no published AI was available, a compound was accepted if the quality of the spectra match was 90% or over.  This project relied upon available linear alkane data from previous projects using the same GC-MS method. The results showed notable AI shifts throughout the 17E data when compared with ‘E’ and other projects. While the AI data remained adequate for confirmation of compounds under the confirmation/rejection parameters outlined above, they were excluded from the phytochemical survey and chromatograms.

Collection: 
A small sample of leaf material (~0.02g) was collected from plants cultivated at the Australian Arid Lands Botanic Garden (Port Augusta, SA) by staff at the garden.  Specimens were immediately placed in glass vials containing a small volume of MeOH and shipped to the lab where they were filtered and MeOH added to make up to ~ 1.5 mL.  Specimens were then injected into the GC-MS for characterization.

GC–MS:
Agilent GC System 7890A; inert MSD with triple-axis detector 7975C; Agilent HP-5MS column (30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min). To limit the impact of solvent-related peaks, peaks prior to a retention time of 3.5 min were not considered in analyses.

Analysis:
Spectra were matched to the NIST11 library and a small private Eremophila compound library maintained at the University of New England. The top 10 peaks were selected, and arithmetic (retention) indices (AI) calculated and compared to the NIST Webbook (http://webbook.nist.gov/chemistry/).  An annotation was rejected if the AI (+/-2%) did not match published values for nonpolar column data.  If the quality match of the spectra to the NIST11 or private library was under 80% the annotation was rejected. Where no published AI was available, a compound was accepted if the quality of the spectra match was 90% or over. 

Collection:
A small sample of dried leaf material (~0.02g) was removed from herbarium vouchers maintained at the N.C.W. Beadle Herbarium (University of New England, Armidale) and placed in glass vials containing ~ 1 mL MeOH.  Material was left to extract overnight as a minimum (up to 2 days) and sonicated for 80 min. Extracts were filtered and diluted with further MeOH as necessary.  Batches were injected into the GC-MS in batches for characterization, or frozen until characterization.

GC–MS:
Agilent GC System 7890A; inert MSD with triple-axis detector 7975C; Agilent HP-5MS column (30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min). To limit the impact of solvent-related peaks, peaks prior to a retention time of 3.5 min were not considered in analyses.

Analysis:
Spectra were matched to the NIST11 library and a small private Eremophila compound library maintained at the University of New England. The top 10 peaks were selected, and arithmetic (retention) indices (AI) calculated and compared to the NIST Webbook (http://webbook.nist.gov/chemistry/).  An annotation was rejected if the AI (+/-2%) did not match published values for nonpolar column data.  If the quality match of the spectra to the NIST11 or private library was under 80% the annotation was rejected. Where no published AI was available, a compound was accepted if the quality of the spectra match was 90% or over. 

Collection:
A small sample of dried leaf material (~0.02g) was removed from herbarium vouchers maintained at the N.C.W. Beadle Herbarium (University of New England, Armidale) and placed in glass vials containing ~ 1 mL MeOH.  Material was left to extract for 24 h, frozen (~-18 °C) and sonicated for 80 min with gentle heating (~50 °C) before being filtered. Extracts were diluted with further MeOH as necessary.  Batches were injected into the GC-MS in batches for characterization, or frozen until characterization.

GC–MS:
Agilent GC System 7890A; inert MSD with triple-axis detector 7975C; Agilent HP-5MS column (30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min). To limit the impact of solvent-related peaks, peaks prior to a retention time of 3.5 min were not considered in analyses.

Analysis:
Spectra were matched to the NIST11 library and a small private Eremophila compound library maintained at the University of New England. The top 10 peaks were selected, and arithmetic (retention) indices (AI) calculated and compared to the NIST Webbook (http://webbook.nist.gov/chemistry/).  An annotation was rejected if the AI (+/-2%) did not match published values for nonpolar column data.  If the quality match of the spectra to the NIST11 or private library was under 80% the annotation was rejected. Where no published AI was available, a compound was accepted if the quality of the spectra match was 90% or over. 

Collection: 
A small sample of leaf material (~0.02g) was collected from plants cultivated at the Australian Arid Lands Botanic Garden (Port Augusta, SA) by staff at the garden.  Specimens were immediately placed in glass vials containing a small volume of MeOH and shipped to the lab where MeOH was added to make up to ~ 1 mL.  Samples were left in the solvent overnight to ~24 h.  Samples were diluted with further MeOH and/or filtered through cotton wool, as necessary. Specimens were then injected into the GC-MS for characterization, or frozen until characterization.

GC–MS:
Agilent GC System 7890A; inert MSD with triple-axis detector 7975C; Agilent HP-5MS column (30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min). To limit the impact of solvent-related peaks, peaks prior to a retention time of 3.5 min were not considered in analyses.

Analysis:
Spectra were matched to the NIST11 library and a small private Eremophila compound library maintained at the University of New England. The top 10 peaks were selected, and arithmetic (retention) indices (AI) calculated and compared to the NIST Webbook (http://webbook.nist.gov/chemistry/).  An annotation was rejected if the AI (+/-2%) did not match published values for nonpolar column data.  If the quality match of the spectra to the NIST11 or private library was under 80% the annotation was rejected. Where no published AI was available, a compound was accepted if the quality of the spectra match was 90% or over. 

Collection: 
A small sample of leaf material (~0.02g) was collected by Mr Phil James from plants cultivated in a private garden (Perth, WA). Specimens were immediately placed in glass vials containing a small volume of MeOH and shipped to the lab where MeOH was added to make up to ~ 1 mL.  Samples were left in the solvent overnight to ~24 h.  Samples were diluted with further MeOH and/or filtered through cotton wool, as necessary. Specimens were then injected into the GC-MS for characterization, or frozen until characterization.

GC–MS:
Agilent GC System 7890A; inert MSD with triple-axis detector 7975C; Agilent HP-5MS column (30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min). To limit the impact of solvent-related peaks, peaks prior to a retention time of 3.5 min were not considered in analyses.

Analysis:
Spectra were matched to the NIST11 library and a small private Eremophila compound library maintained at the University of New England. The top 10 peaks were selected, and arithmetic (retention) indices (AI) calculated and compared to the NIST Webbook (http://webbook.nist.gov/chemistry/).  An annotation was rejected if the AI (+/-2%) did not match published values for nonpolar column data.  If the quality match of the spectra to the NIST11 or private library was under 80% the annotation was rejected. Where no published AI was available, a compound was accepted if the quality of the spectra match was 90% or over. 

Collection: 
A small sample of leaf material (~0.02g) was collected by Dr Bevan Buirchell from plants cultivated in a private garden (Goomalling, WA). Specimens were immediately placed in glass vials containing a small volume of MeOH and shipped to the lab where MeOH was added to make up to ~ 1 mL.  Samples were left in the solvent overnight to ~24 h.  Samples were diluted with further MeOH and/or filtered through cotton wool, as necessary. Specimens were then injected into the GC-MS for characterization, or frozen until characterization.

GC–MS:
Agilent GC System 7890A; inert MSD with triple-axis detector 7975C; Agilent HP-5MS column (30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min). To limit the impact of solvent-related peaks, peaks prior to a retention time of 3.5 min were not considered in analyses.

Analysis:
Spectra were matched to the NIST11 library and a small private Eremophila compound library maintained at the University of New England. The top 10 peaks were selected, and arithmetic (retention) indices (AI) calculated and compared to the NIST Webbook (http://webbook.nist.gov/chemistry/).  An annotation was rejected if the AI (+/-2%) did not match published values for nonpolar column data.  If the quality match of the spectra to the NIST11 or private library was under 80% the annotation was rejected. Where no published AI was available, a compound was accepted if the quality of the spectra match was 90% or over. 

Collection: 
A small sample of leaf material (~0.02g) was collected by Mr Russell Wait from plants cultivated in a private garden (Riddells Creek, VIC). Specimens were immediately placed in glass vials containing a small volume of MeOH and shipped to the lab where MeOH was added to make up to ~ 1 mL.  Samples were left in the solvent overnight to ~24 h.  Samples were diluted with further MeOH and/or filtered through cotton wool, as necessary. Specimens were then injected into the GC-MS for characterization, or frozen until characterization.

GC–MS:
Agilent GC System 7890A; inert MSD with triple-axis detector 7975C; Agilent HP-5MS column (30 m × 250μm× 0.25μm; He; 50 °C for 0 min, 5 °C/min to 260 °C, hold 2 min). To limit the impact of solvent-related peaks, peaks prior to a retention time of 3.5 min were not considered in analyses.

Analysis:
Spectra were matched to the NIST11 library and a small private Eremophila compound library maintained at the University of New England. The top 10 peaks were selected, and arithmetic (retention) indices (AI) calculated and compared to the NIST Webbook (http://webbook.nist.gov/chemistry/).  An annotation was rejected if the AI (+/-2%) did not match published values for nonpolar column data.  If the quality match of the spectra to the NIST11 or private library was under 80% the annotation was rejected. Where no published AI was available, a compound was accepted if the quality of the spectra match was 90% or over.